Fig. 3. cHA-LAIV vaccines induce robust serum and mucosal responses against group 1 stalk and confer ADCC reporter assay activity.

Longitudinal area-under-the-curve values (AUCs) were calculated using endpoint titers from serum/nasal washes derived from mice before, between and after immunization. a Mucosal total IgA titers depicted as AUC values for group 1 HA stalk derived from endpoint titers of (n = 3) from animal nasal washes (undiluted starting dilution) on D98. b Longitudinal group 1 HA stalk total IgG titers from pooled sera. c Individual values of group 1 HA stalk total IgG titers from serum on D98. d ADCC reporter assay activity from serially diluted serum samples (1:20 starting dilutions) in which endpoint titers were used to calculate area under the curve values (AUCs) plotted from 3 sets of pooled animal serum from each respective group. In (a), significance established by comparisons against the group with the highest values (Group 1) while all other panels had comparisons to QIV standard of care group (Group 7) using one way-ANOVA for multiple comparisons corrected and adjusted as per Dunnett’s correction. ****P- < 0.0001, ***P-0.001, **P-0.002, *P-0.02. Data are shown as mean ± SEM. Limits of detection (LOD) is shown in dotted lines. Upper dotted line indicates HA-Stalk Prime only (Group 5) mean.