Fig. 6. cHA-ΔNS1-LAIV vaccination induces potent antigen-specific cytotoxic CD8 T-cell responses during virus challenge.

Four weeks post-final-boost, mice (n = 5 per group) were challenged with a QIV-matched IVR-180 using a lethal dose of 100x LD₅₀. a A representative image of the gating strategy used to probe NP-tetramer+ CD8⁺ T-cell responses of pooled blood derived from mice on D1, D5, and D10 post-challenge. (The same animals were followed throughout the study). The following markers were utilized for the gating strategy; CD3e – T cell marker, CD8a – CD8 T cell marker. b Amino-acid sequence of the NP tetramer used in this study corresponds to all the NPs of the viruses used in this study. c Longitudinal assessment of IAV-NP⁺CD8⁺ circulating T-cells expressed as a percentage of total CD8⁺ T-cells (left) and percentage of total lymphocytes (right).