Fig. 8. ELISpot assays for IFNγ⁺CD8⁺ T-cells from splenocytes indicate robust T-cell responses against viral NP and HA stalk, induced by cHA-LAIV vaccinations.

Four weeks post-final-boost, mice were challenged with a QIV-matched IVR-180 using a lethal dose of 100x LD_50. On day 5 post-infection, spleens were harvested and processed for ELISpot assays. Splenocytes (10⁵ cells) were stimulated with indicated CD8 restricted peptides for 16 h. a HA stalk-peptide stimulated. b NP peptide stimulated and c. RSV-F peptide stimulated (irrelevant peptide). Each dot represents one animal. Side panels indicate representative ELISpot images form each animal. Data present as mean ± SEM. Statistical significance was compared to QIV standard of care group (Group 7) using one-way ANOVA using Dunnett’s-correction. ****P- <0.0001, ***P-0.0002, **P-0.0011, *P-0.01. Limits of detection (LOD) are shown in dotted lines.