FIG. 2.

T-lymphocyte and neutrophil infiltration of the eyes of CVS-infected mice. The nature of infiltrating cells was determined in the vitreous humor, retina, and cornea of mock-infected and CVS-infected mice 10 days p.i. Inflammatory cells were detected by Hoechst blue staining in the IPL of the eyes from CVS-infected C57BL/6 mice (B) but not in those from mock-infected mice (A). Frozen eye sections obtained from mock-infected mice (C, E, G, and I) and CVS-infected mice (D, F, H, and J) were incubated in the presence of FITC-conjugated MAb specific for Gr-1 (C and D) or FITC-conjugated MAb specific for CD3 (E to J). Infiltrating neutrophils and T lymphocytes were located mainly in the GCLs, IPLs, and INLs of the retinas of CVS-infected mice (D), but T lymphocytes were also present in the vitreous humor (F), the ciliary body (H), and the cornea (J). VITR, vitreous cavity; OPL, outer plexiform layer; ONL, outer nuclear layer. Bars, 40 μm (A and B), 20 μm (C to F), and 100 μm (G to J).