FIG. 5.

Most of the infiltrated CD3⁺ T cells are not apoptotic in the eyes of CVS-infected mice. Apoptosis was detected by the TUNEL technique in the retinas of mock-infected (A) and CVS-infected (B to D) mice. No TUNEL-positive cells were detected in mock-infected mice. Also shown is codetection of apoptosis (TUNEL red) and Gr-1-positive cells (B) or CD3-positive cells (green) in the retinas (C) and vitreous cavities (D) of CVS-infected mice 10 days p.i. (B and C) TUNEL-positive cells (white arrows) are mainly photoreceptors and do not correspond to inflammatory cells (open arrows). (D) Both apoptotic (white arrow) and nonapoptotic (open arrows) CD3-positive cells were observed. OPL, outer plexiform layer; ONL, outer nuclear layer; RPE, retinal pigmented epithelium. Bars, 50 μm (A and B), 25 μm (C), and 10 μm (D).