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. 2024 Sep 6;12:1434269. doi: 10.3389/fcell.2024.1434269

FIGURE 5.

FIGURE 5

NOD.B10 females exhibit heightened immune activation in secondary lymphoid tissues as compared to males and ablation of Tlr7 in males and females has dichotomous effects. Spleens and cLNs were harvested from NOD.B10 female (n = 11) and male mice (n = 8) and from NOD.B10 Tlr7+/− (n = 9), NOD.B10 Tlr7−/− (n = 13), and NOD.B10 Tlr7-/y mice (n = 9). Flow cytometry was performed to quantify the following immune populations in the spleen: (A) Fo B cells (B220+ CD23+CD21lo/-), (B) MZ B cells (B220+ CD23− CD21+), (C) T-bet + ABCs (B220+ CD23− CD21− T-bet+ CD11c+), (D) T-bet+ CD11c+ ABCs (B220+ CD23− CD21− T-bet+ CD11c+) (E) GC B cells (B220+ Fas + GL7+), (F) Plasmablasts (B220+ CD138+), (G) Activated/memory (Act/Mem) CD4+ T cells (CD4+ CD44+ CD62L-) and (H) Activated/memory CD8+ T cells (CD8+ CD44+ CD62L-). Similar populations were quantified in cLNs as follows: (I) GC B cells, (J) Plasmablasts (K) Activated/memory CD4+ T cells, and (L) Activated/memory CD8+ T cells. Horizontal lines represent mean and SEM, (*p < 0.05, **p < 0.01 for the hypothesis tests described in the statistical section).