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. 2024 Sep 6;12:1434269. doi: 10.3389/fcell.2024.1434269

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Copyright © 2024 Punnanitinont, Biswas, Kasperek, Osinski, Zhu, Miecznikowski, Romano and Kramer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tlr7 and Tlr9 expression levels are altered in pSD females as compared to males. Spleens were harvested from NOD.B10 female and male mice. Flow cytometry was performed to quantify the (A) percentage of Tlr7-expressing B cells and B cell Tlr7 MFI in NOD.B10 females (n = 11) and males (n = 9) and (B) the percentage of Tlr9-expressing B cells and Tlr9 MFI in NOD.B10 (n = 10) and NOD.B10^Tlr7−/− females (n = 10) and NOD.B10 (n = 10) and NOD.B10^Tlr7y/- males (n = 7). Horizontal lines represent mean and SEM, (*p < 0.05, **p > 0.01 for the hypothesis tests described in the statistical section).