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. 2024 Aug 26;14(14):5413–5428. doi: 10.7150/thno.97269

Figure 2.

Figure 2

Biodistribution of mEVs and histological analysis of the interaction between FcRn and mEVs in the intestinal region. (A) In vivo whole-body NIRF images after oral administration of Free dye, FDX, mEVs, and FDX@mEVs at various time points (20, 40 min, 1, 3, 6, and 24 h) and ex vivo NIRF images of major organs (liver (Li), lungs (Lu), spleen (Sp), kidneys (Ki), and heart (He) including the GI tract. (B) Remaining % fluorescence signals of Free dye, FDX, mEVs, and FDX@mEVs in GI tract in (A). (C) Quantified fluorescence intensity of Free dye, FDX, mEVs, and FDX@mEVs expressed as the radiant efficiency in the GI tract in ex vivo images. (D) Histological analysis of Free dye, FDX, mEVs, FDX@mEVs, and FcRn in intestinal villi. Scale bar: 50 μm. (E) Colocalization analysis between FcRn (green) and mEVs (red) signals. All data presented as mean ± SD, One-way ANOVA with Tukey's post-hoc multiple comparisons, *P < 0.05 and **P <0.01, ***P < 0.001, ****P < 0.0001, #P < 0.05, ##P < 0.01, and ####P < 0.0001. The asterisk (*) indicates significance compared to the Free dye group, while the hash (#) denotes significance compared to FDX.