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. 2024 Sep 20;43:264. doi: 10.1186/s13046-024-03158-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Combined analyses of cfDNA and ctDNA identify the patients with the worst clinical outcome. A-B. Kaplan Meier curves showing DFS (A) and DSS (B) in patients according to the pre-surgery high levels of cfDNA and detectable levels of ctDNA. C. Bar plot with the early recurrence status according to the combinatorial approach and the ESGO risk classification D. Graphical representation of the univariate (blue) and multivariate (red) Cox proportional-hazard models. P-value > 0.05 is represented with the *symbol. E-F. Kaplan-Meier curves showing DFS in patients according to the pre-surgery high levels of cfDNA and detectable levels of ctDNA in patients with low or intermediate (E) and high-intermediate or high (F) risk of recurrence based on the ESGO-ESTRO-ESP risk stratification