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. 2024 Sep 20;22:848. doi: 10.1186/s12967-024-05616-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overview of the established workflow. Depicted is the established workflow for isolating and analyzing single CTCs with Reverse Phase Protein Arrays (RPPA). The workflow consists of three primary steps: (A) Capture and verification of CTCs using Diagnostic Leukapheresis (DLA) combined with the CellSearch® system. (B) Single cell/CTC picking with a glass capillary, followed by in situ lysing and mixing on a Micropick 48 slide™ and subsequent printing onto ZeptoChip using a modified CellCelector™ instrument. (C) RPPA analysis of the chip using the ZeptoReader