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. 2024 Sep 20;22:848. doi: 10.1186/s12967-024-05616-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Optimization of conditions and hardware for single cell lysis and manipulation. (A) Monitoring of single cell lysis efficiency upon variation of lysis buffer composition. For better visualization, MCF-7 cells were prelabeled with Celltracker Green and NucBlue™ Live ReadyProbes™, and fluorescence images under the microscope were taken at indicated time points during lysis; Scale bar: 25 μm, Magnification:10x; DAPI (nucleus) and FITC (cytoplasm); (B) Lysis efficiency: comparison of complete lysis durations (min) for different CLB1:CSBL1 ratios; (C) Design of the holder for the ZeptoChip (left) and the adapter for the Micropick 48® slide, the Magnetpick® slide, and for the ZeptoChip (plus its holder) to position them precisely onto the CellCelector’s microscope stage (right)