Figure 4.

Directly binding between Cel and VDAC1. (A) Predicted binding sites of VDAC1 with Cel by Autodock. Yellow dotted lines represent hydrogen bonds, and purple sticks represent Cel compounds. (B) Cel-p and (C) IAA-yne labeling of recombined human VDAC1 protein (1-2 µg) in a dose-dependent manner. (D) Cel-p and (E) IAA-yne labeling of recombined human VDAC1 protein was done in the presence or absence of corresponding competitors. (F) Chemical changes of Cel upon binding with targets using a model presentation. Cel celastrol, IAA iodoacetamide, VDAC1 voltage-dependent anion-selective channel protein 1, Fluo fluorescence, CBB coomassie brilliant blue, Conc. concentration, IAA iodoacetamide, CYS cystine, THR threonine, ILE isoleucine.