Figure 8.

Potential mechanism of Cel-induced nephrotoxicity (schematic). (A) Cel sources and its nephrotoxicity in vivo and in vitro. (B) Strategy combining ABPP and CETSA to identify and validate the binding targets of Cel on HK-2 cells and kidney tissues. (C) Cel targeted the proteins (enzymes) associated specifically with metabolism, ultimately resulting in mitochondrial dysfunction and apoptosis. This figure was drawn using www.biorender.com. Cel celastrol, ABPP activity-based protein profiling, CETSA cellular thermal shift assay.