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. 2024 Sep 16;20(12):4922–4940. doi: 10.7150/ijbs.87829

Figure 1.

Figure 1

TRPC5 is functionally overexpressed in gastrointestinal cancer. (A) Representative HE and TRPC5 IHC staining images from clinical gastric and colorectal cancer samples (n = 5 for STAD, n = 13 for COAD). Scale bar: 200 μm. (B) Quantitative analysis of TRPC5 IHC staining levels between tumors and adjacent normal tissues (P < 0.001 vs. adjacent normal tissues). (C) Relative TRPC5 mRNA expression in indicated gastric and colorectal cancer cells. Data are expressed as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 (vs. GES-1 or NCM460 cells). (D, E) Immunoblot analysis of TRPC5 protein expression in the indicated gastric and colorectal cancer cells. Data are expressed as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 (vs. GES-1 or NCM460 cells). (F) Representative IF staining of TRPC5 in GES-1, MKN-45, NCM460, and DLD-1 cells (blue: nuclei, red: F-actin, green: TRPC5). Scale bar: 50 μm. (G) Averaged time courses of Ca2+ imaging (F340/380) and representative traces of calcium responses in gastric and colorectal cancer cells induced by 50 μM Gd3+. (H) Maximal [Ca2+]i increases (ΔF340/380) in gastric and colorectal cancer cells evoked by 50 μM Gd3+ (n = 10). Data are expressed as means ± SD. (I) The Pearson correlation analysis revealed a strong linear relationship between the expression of TRPC5 and the maximal rise in intracellular Ca2+ concentration (ΔF340/380), with a correlation coefficient of 0.9399351.