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. 2024 Sep 9;15(17):5762–5772. doi: 10.7150/jca.99610

Figure 2.

Figure 2

SCAMP1 knockdown markedly attenuated the proliferation of GC cells in vitro and in vivo. (A) The mRNA and protein levels of endogenous SCAMP1 in GES-1 cells and a panel of GC cell lines. The difference in SCAMP1 mRNA expression between GES-1 cells and each GC cell line was evaluated by using one-way ANOVA with Dunnett's post hoc test, which demonstrated that SCAMP1 mRNA levels in all GC cell lines were significantly increased relative to that in GES-1 cells (*, P < 0.05; ****, P < 0.0001). (B) Immunoblotting was used to validate the efficacy of endogenous SCAMP1 knockdown in NCI-N87 (left) and SNU-1 (right) cells. (C) CCK-8 assays demonstrated that SCAMP1 depletion reduced the viability of NCI-N87 (left) and SNU-1 (right) cells. Statistical significance was determined using one-way ANOVA with Dunnett's post hoc test. (D) EdU incorporation assays showed that the proportions of replicating SCAMP1-depleted NCI-N87 (left) and SNU-1 (right) cells decreased markedly. One-way ANOVA with Dunnett's post hoc test was used to assess statistical significance. (E and F) SCAMP1 knockdown diminished the proliferation of NCI-N87 cells in vivo, as demonstrated by reduced tumor volumes (n = 6) (E) and decreased tumor weights (n = 6) (F). Paired t-test was used to evaluate statistical significance in E and F. **, P < 0.01; ***, P < 0.001.