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. 2024 Sep 9;15(17):5762–5772. doi: 10.7150/jca.99610

Figure 3.

Figure 3

Depletion of SCAMP1 deactivated multiple cancer-promoting signaling pathways. (A) RNA-seq demonstrated that the expression of 495 genes increased while the expression of 204 genes decreased in shS1#1 NCI-N87 cells compared with shCtrl NCI-N87 cells. (B) GO analysis showed that the differentially expressed genes (DEGs) were primarily enriched in membrane receptors and their implicated signaling pathways. (C) The GC cohort in TCGA was stratified into low-SCAMP1-expression and high-SCAMP1-expression groups, and the DEGs between these two groups were also enriched in signaling pathways similar to those revealed by RNA-seq. (D) Immunoblotting assays showing that SCAMP1 knockdown markedly reduced the levels of p-Akt (Ser473), p-ERK1/2 (Thr202/Tyr204), and p-STAT3 (Tyr705). (E) The ratios of p-AKT/AKT, p-ERK/ERK, p-STAT3/STAT3, and SCAMP/vinculin remarkably decreased in shS1#1/#2 cells relative to those in shCtrl cells. Two-way ANOVA with Bonferroni post hoc test was conducted to evaluate the statistical significance. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.