Figure 1.

eIF2B localization is cell-type specific

(A) SH-SY5Y, U373, and MO3.13 cells subjected to transient transfection and expressing eIF2Bε-mGFP. Scale bar: 10 μm.

(B) (i) Cells express dispersed eIF2B or localized eIF2B (eIF2B bodies). (ii) Mean percentage of cells displaying dispersed eIF2B or localized eIF2B in a population of 100 transfected cells (mean ± SEM; N = 4; ∗∗∗∗p ≤ 0.001, ∗∗∗p ≤ 0.001 according to two-way ANOVA).

(C) (i) eIF2B bodies were categorized as small bodies (<1μ²) and large bodies (≥1μ²). (ii) Within the transfected cells exhibiting localized eIF2B, the mean percentage of small and large eIF2B bodies in a population of 50 cells (mean ± SEM; N = 3; ∗∗∗∗p ≤ 0.001, ∗∗∗p ≤ 0.001, ns: non-significant according to two-way ANOVA).

(D) (i) Confocal images of SH-SY5Y, U373 and MO3.13 expressing eIF2Bε-mGFP and immunolabelled with primary anti-eIF2Bα, anti-eIF2Bβ, anti-eIF2Bδ and anti-eIF2Bγ. Scale bar: 10 μm. (ii) Mean percentage of small (top panel) and large bodies (bottom panel) co-localizing with eIF2B(α-γ) subunits of at least 30 cells per repeat (mean ± SEM; N = 3; ∗∗p ≤ 0.01, ∗p < 0.05 according to one-way ANOVA).