REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-eIF2Bα	Proteintech	Cat# 18010-1-AP; RRID: AB_2261996
Rabbit polyclonal anti-eIF2Bβ	Proteintech	Cat# 11034-1-AP; RRID: AB_2096016
Mouse monoclonal anti-eIF2Bγ	Santa Cruz Biotechnology	Cat# sc-137248; RRID: AB_2096150
Mouse monoclonal anti-eIF2Bδ	Santa Cruz Biotechnology	Cat# sc-271332; RRID: AB_10610502
Rabbit polyclonal anti-eIF2Bε	Sigma-Aldrich	Cat# HPA069303; RRID: AB_2686114
Mouse monoclonal anti-eIF2α	Abcam	Cat# ab5369; RRID: AB_304838
Rabbit recombinant monoclonal anti-eIF2α[ser51-P] [E90]	Abcam	Cat# ab32157; RRID: AB_732117
Rabbit polyclonal anti-PERK	Proteintech	Cat# 20582-1-AP; RRID: AB_10695760
Rabbit polyclonal anti-GADD34	Proteintech	Cat# 10449-1-AP; RRID: AB_2168724
Rabbit polyclonal anti-CHOP	Proteintech	Cat# 15204-1-AP; RRID: AB_2292610
Rabbit monoclonal anti-ATF4 (discontinued)	Abcam	Cat# ab184909; RRID: AB_2819059
Rabbit monoclonal anti-GAPDH	Cell Signaling Technology	Cat# 2118; RRID: AB_10693448
Mouse monoclonal anti-puromycin (clone 12D10)	Merck Millipore	Cat# MABE343; RRID: AB_2566826
Mouse anti-GFAP	This work	N/A
Rabbit polyclonal anti-MBP	Abcam	Cat# ab40390; RRID: AB_1141521
Mouse monoclonal anti-NeuN	Abcam	Cat# ab104224; RRID: AB_10711040
Goat anti-rabbit IRDye 680RD	LI-COR	Cat# 925–68071; RRID: AB_2721181
Goat anti-mouse IRDye 800CW	LI-COR	Cat# 925–32210; RRID: AB_2687825
Goat anti-Rabbit Alexa Fluor 594®	Invitrogen	Cat# A-11012; RRID: AB_2534079
Goat anti-Mouse Alexa Fluor 594®	Invitrogen	Cat# A-11032; RRID: AB_2534091
Goat anti-Mouse Alexa Fluor 488®	Invitrogen	Cat# A-11001; RRID: AB_2534069
Goat anti-Rabbit Alexa Fluor 488®	Invitrogen	Cat# A-11008; RRID: AB_143165
Chemicals, peptides, and recombinant proteins
Thapsigargin (Tg)	Sigma-Aldrich	Cat# T9033
Sodium arsenite (SA)	Sigma-Aldrich	Cat# S7400
Tunicamycin (Tm)	Cayman Chemical	Cat# 11445
GSK2606414 (PERK inhibitor)	Tocris	Cat# 5107
ISRIB	Sigma-Aldrich	Cat# SML0843
Puromycin dihydrochloride	Gibco	Cat# A1113803
Cycloheximide	Merck Millipore	Cat# 239763
Branched 25-kDa polyethylenimine (PEI)	Sigma-Aldrich	Cat# 408727
CelLytic M	Sigma-Aldrich	Cat# C2978
Phosphatase inhibitor cocktail 2	Sigma-Aldrich	Cat# P5726
Phosphatase inhibitor cocktail 3	Sigma-Aldrich	Cat# P0044
Protease inhibitor cocktail	Sigma-Aldrich	Cat# P8340
4x Laemmli sample buffer	Bio-Rad	Cat# 1610747
Critical commercial assays
Lipofectamine™ 3000 Transfection Reagent	Invitrogen	Cat# L3000001
Qubit™ Protein Assay kit	Invitrogen	Cat# Q33212
Revert 700 Total Protein Stain	LI-COR	Cat# 926-11011
Deposited data
Original immunoblots	Mendeley Data Repository	https://doi.org/10.17632/tjtcftj3cf.1
Experimental models: Cell lines
SH-SY5Y	ATCC	Cat# CRL-2266
U373	Sigma-Aldrich	Cat# 08061901
MO3.13	Cedarlene	Cat# CLU301-P
Recombinant DNA
pCMV6-AC-mGFP	Origene	Cat# PS100040
pCMV6-AC-mRFP	Origene	Cat# PS100034
Plasmid: EIF2B5-tGFP	Origene	Cat# RG202322
Plasmid: EIF2S1-tGFP	Origene	Cat# RG200368
Plasmid: EIF2B5-mGFP	This work	N/A
Plasmid: EIF2B5-mRFP	This work	N/A
Software and algorithms
GraphPad Prism 9.2.0	GraphPad	https://www.graphpad.com/
Zeiss Zen 2.3 (Blue edition)	Zeiss	https://www.zeiss.com/
Image Studio™ Lite	LI-COR	https://www.licor.com/bio/image-studio/
Illustrator CS6	Adobe Inc.	https://www.adobe.com/products/illustrator.html
BioRender	BioRender	https://www.biorender.com/