Fig. 2.

Hypoxia promotes Hmox1 expression in MDFCs and drives ferroptotic cell death.

A. Volcano plot of DEGs in GSE16099 and enrichment analysis (GO enrichment analysis and KEGG pathway analysis). B. Schematic representation of BMDM isolation and foam cell formation. C. Western blot analysis of macrophages and ox-LDL-treated macrophages (MDFCs) in the presence or absence of hypoxia. D. CCK-8 assays were used to assess the effects of different inhibitors on MDFC activity under hypoxia. E. The relative GSH/GSSG ratios were assayed. F. Iron and lipid peroxidation levels of macrophages and MDFCs in response to hypoxia. Scale bar, 50 μm. MDFC, macrophage-derived foam cell; DEGs, differentially expressed genes; BP, biological process; CC, cellular component; MF, molecular function; ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.