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. 2024 Sep 20;10(38):eadj4122. doi: 10.1126/sciadv.adj4122

Fig. 4. SELENOW deletion aggravates age-related sarcopenia.

Fig. 4.

(A) Schematic showing the chronic sarcopenia mice model established by raising C57 mice from 3 months to 22 to 24 months. (B) Average body weights of mice from 3 to 24 months of age, n = 13 to 15. (C and D) Grip strength and hanging time of C57 mice from 22 to 24 months of age, n = 8 to 9. (E and F) Gross morphology mass analysis of TA, EDL, GAS, SOL, and QU in aged mice after DEX treatment, n = 18 to 22. (G to J) MDA, GSH, GPX4, and TXNRD concentration or activity in GAS muscle, n = 13 to 15. (K) Representative H&E staining and Laminin staining of myofiber cross section of TA muscle; scale bars, 100 μm. (L and M) Related myofiber CSA and frequency of distribution for CSA of TA muscle a, n = 7. (N and O) Representative Laminin staining of type IIA fibers in TA muscle; scale bar, 100 μm. (O) Related CSA of type IIA fibers in TA muscle, n = 5. (P to S) Western blot analysis of protein levels of MyHC, Atrogin-1, and MuRF-1 in TA and GAS muscle. GAPDH was used as the loading control, n = 4. Data are means ± SEM; Student’s t test, *P ≤ 0.05, **P ≤ 0.01.