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. 2024 Sep 20;10(38):eadj4122. doi: 10.1126/sciadv.adj4122

Fig. 5. SELENOW functions in sarcopenia may via Rho signaling.

Fig. 5.

(A) Volcano diagram of DEGs in TA muscle between WT and KO aged mice, n = 3. (B) Heatmap diagram showing the cluster analysis of DEGs in TA muscle of WT and KO aged mice, n = 3. (C) GO analyses showing the enrichment of functional categories in TA muscle of WT and KO aged mice, n = 3 mice. (D and E) Heatmap diagram showing the up-regulated genes related to small Rho protein signaling translation and down-regulated genes related to muscle system processes in TA muscle of WT and KO mice, n = 3. (F) qPCR analysis of the relative mRNA expression of Rho transduction related gene (ARHGEF40, EPS8L2, FGD5, MCF2L, and TIAM1) and muscle system-related genes (MYOD, MYOG, CHRNG, MYL4, MYOZ2, P2RX4, and TNNC1), n = 6. (G) Co-IP analysis the protein interaction between Rho protein (RAC1 and CDC42) and SELENWO. (H) Laser confocal showing the cellular localization of RAC1 and SELENOW in C2C12 cells. (I) The binding mode prediction between SELENOW and RAC1. Data are means ± SEM; Student’s t test, *P ≤ 0.05, ***P ≤ 0.001.