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Comparison of Western blot reactivity between NRA produced by pCBWN-transformed COS-1 cells (A) and gradient-purified WN virion proteins (V). WN virus-specific mouse HIAF, flavivirus E-specific cross-reactive MAb 4G2, and eastern equine encephalitis virus monoclonal antibody (EEE MAb) were used at a 1:200 dilution in the assay. Sizes are shown in kilodaltons.
