FIG. 1.
SDS-PAGE analysis of viral proteins synthesized in ts25-infected cells. L929 cells were infected with either the WR or ts25 strain of vaccinia virus and incubated at the permissive (A) or nonpermissive (B) temperature in the presence or absence of AraC. Cells were labeled with [35S]methionine for 30 min at the indicated times (hours postinfection [hpi]). Lysates were prepared, immunoprecipitated with anti-vaccinia virus antiserum, and analyzed by SDS-PAGE. Masses of protein standards in kilodaltons are indicated to the left. U, uninfected; MW, molecular weight standards.