FIG. 5.

Expression of reporter genes in cells infected with ts25T7. (A) L929 cells were infected with ts25T7 at the indicated temperatures in the presence or absence of AraC. Lysates were prepared at 24 h after infection and analyzed by Western blotting using an anti-T7 RNA polymerase antibody. A 97.4-kDa protein standard (MWM) and the expected position of T7 RNA polymerase (denoted by the arrow) are indicated on the left and right, respectively. (B and C) L929 cells were transfected with pG8R-CAT or pT7-CAT and infected with either ts25T7 or VTF7.3 at the indicated temperatures. Extracts were prepared at 18 h after infection and assayed for CAT activity (expressed as percent acetylation).