FIG. 2.

Detection of DNA content of rSV5- and rSV5ΔSH-infected MDBK cells using propidium iodide staining. MDBK cells in 6-cm plates were infected and processed for propidium iodide staining of DNA 1 or 3 days p.i. as described in Materials and Methods. (A) DNA staining of 10,000 cells was detected by flow cytometry. “FL2-A” indicates DNA content; “A” indicates sub-G₀-G₁ DNA content, which is considered apoptotic. (B) Histogram of data computed from three samples like those shown in panel A for each time point and virus.