FIG. 3.

TUNEL assay of rSV5- and rSV5ΔSH-infected MDBK cells. MDBK cells were mock infected or infected with rSV5 or rSV5ΔSH and at 1 or 3 days p.i. were processed for the TUNEL assay as described in Materials and Methods. (A) Detection by flow cytometry of fluorescently labeled DNA. The area marked “Apoptotic” indicates fluorescence intensity of cells considered positive for the TUNEL assay and hence apoptotic. (B) Histogram of three independent experiments as in panel A at each time point.