Fig. 3.
Two doses of 71VG-adjuvanted RG6 confer protection against H5N1 VN clade 1 virus but exhibit limited cross-reactivity against clade 2.3.4.4 H5 viruses Chickens vaccinated with 2 doses of RG6 were challenged with a 10 × LD50 of H5N1 VN (Clade 1) after day 28 post-vaccination. The Kaplan-Meier method presents the survival curves. The significant difference in the survival outcome between sham-vaccinated (black circles) and RG6-vaccinated (blue circles) chickens was assessed by the Log-Rank test (p-value < 0.005). (B) The HI antibody titers against homologous RG6 antigen or against viral antigens from H5N1 VN Clade 1 and GV20686, GV20018, CV20623, and CV20629 (Clade 2.3.4.4c) were obtained for 12 antiserum samples from chickens vaccinated with RG6 (Clade 2.3.4) at day 28. Each symbol represents an individual animal. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)