Chemical quench results of the AsqJ reaction with 1-H at different reaction times. The LC-MS analysis revealed the sequential formation of 2-H and 3-H. The figure is adapted with permission from Liao, H.-J., Li, J., Huang, J.-L., Davidson, M., Kurnikov, I., Lin, T.-S., … Chang, W. (2018). Insights into the desaturation of cyclopeptin and its C3 epimer catalyzed by a non-heme iron enzyme: Structural characterization and mechanism elucidation. Angewandte Chemie International Edition, 57(7), 1831–1835. https://doi.org/10.1002/anie.201710567 Copyright 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.