Table 1.
Preparation of crystallization drops for AsqJ•Fe•succinate•1-H and AsqJ•Fe•2OG•2-CF3 complexes using the hanging drop method.
| AsqJ•Fe•succinate•1-H complex |
AsqJ•Fe•succinate•2-CF3 complex |
|
|---|---|---|
| AsqJ•Fe•succinate•1-H (protein sample, μL) | 0.1 | – |
| AsqJ•Fe•succinate•2-CF3 (protein sample, μL) | – | 0.1 |
| ddH2O (μL) | 0.2 | – |
| reservoir solution (20% (w/v) PEG 1000, 100 mM imidazole/HCl, pH 8.0, 0.2 M calcium acetate) (μL) | 0.1 | – |
| reservoir solution (either 1.2 M potassium sodium tartrate tetrahydrate or 0.6 M potassium sodium tartrate tetrahydrate, both with 0.1 M Tris, pH 8.5) (μL) | – | 0.1 |
| Total drop volume (μL) | 0.4 | 0.2 |