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Preparation of AsqJ•Fe•2OG•1-H and AsqJ•Fe•2OG•D-1-H crystals by soaking pre-grown AsqJ•Fe•succinate•1-H crystals.
| AsqJ•Fe•2OG•1-H complex | AsqJ•Fe•2OG•D-1-H complex | |
|---|---|---|
| Pre-grown crystal | AsqJ•Fe•succinate•1-H | AsqJ•Fe•succinate•1-H |
| Soaking solution | 25% PEG 1000, 100 mM imidazole/HCl (pH 8.0), 10 mM 2OG, 2 mM 1-H | 25% PEG 1000, 100 mM imidazole/HCl (pH 8.0), 10 mM 2OG, 2 mM D-1-H |
