Table 2.
Primer sequences used in real-time quantitative PCR.
| Genes | Accession No. | Position | Primer (5′ - 3′) |
|---|---|---|---|
| IL-1β | NM_214055.1 | Forward Reverse |
TCTGCCCTGTACCCCAACTG CCAGGAAGACGGGCTTTTG |
| IL-6 | AF518322.1 | Forward Reverse |
TGGCTACTGCCTTCCCTACC AGAGCCTGCATCAGCTCAGT |
| IL-12 | NC_010458.4 | Forward Reverse |
CAACCCTGTGCCTTAGCAGT AGAGCCTGCATCAGCTCAGT |
| TNF-α | NM_214022.1 | Forward | CCACCAACGTTTTCCTCACT |
| Reverse | TAGTCGGGCAGGTTGATCTC | ||
| TGF-β | NM_001087861.1 | Forward | ATTCCTGGCGTTACCTTGG |
| Reverse | AGCCCTGTATTCCGTCTCTCCT | ||
| TLR4 | NM_001113039.1 | Forward | CAACCCTGTGCCTTAGCAGT |
| Reverse | AGAGCCTGCATCAGCTCAGT | ||
| MyD88 | NM_001099923 | Forward | CGCATGGTGGTGGTTGTT |
| Reverse | GCCTTCTTCATCGCCTTGTATTT | ||
| IKKα | NM_001114279.1 | Forward | TCTTGATCCTCGGAAACCAG |
| Reverse | TGCTTCGGCCCATACTTTAC | ||
| IKKβ | NM_001099935.1 | Forward | CCTCACCTTGCTGAGTGACA |
| Reverse | TCCCCACAAAGGAGGTACAG | ||
| IκB | EU399817.1 | Forward | CTGCTCGGCAATAACACTGA |
| Reverse | GAGAGGAGACCGTTGGTGAG | ||
| ERK | NM_021088019.1 | Forward | CAGTCTCTGCCCTCCAAGAC |
| Reverse | GGGTAGATCATCCAGCTCCA | ||
| JNK | NM _001929166.6 | Forward | TGGATGAAAGGGAACACACA |
| Reverse | ATGATGACGATGGATGCTGA | ||
| P38 | NM_021091323.1 | Forward | AAGACGGGGTCCTCATCTCC |
| Reverse | TCTCATCGTAGGGCTCTGCT | ||
| NFKB | NM_001114281.1 | Forward | GGGGCGATGAGATCTTCCTG |
| Reverse | CACGTCGGCTTGTGAAAAGG | ||
| β-Actin | XM_021086047.1 | Forward | GATCTGGCACCACACCTTCTACAAC |
| Reverse | TCATCTTCTCACGGTTGGCTTTGG |
IL-1β = interleukin-1 beta; IL-6 = interleukin-6; IL-12 = interleukin-12; TNF-α = tumor necrosis factor-alpha; TGF-β = transforming growth factor-beta; TLR-4 = Toll-like receptor 4; MyD88 = myeloid differentiation primary response gene 88; IKKα = IκB kinase α; IKKβ = inhibitor of kappa B kinase; IκB = inhibitor of NF-κB; ERK = extracellular regulated protein kinases; JNK = c-Jun N-terminal kinase; P38 = p38 mitogen-activated protein kinase; NF-κB = nuclear factor-kappa B.