FIG. 2.

Repression of HPV31 P97 activity by E8̂E2C in NHKs does not require promoter-proximal E2 binding sites. NHK were cotransfected with different HPV31 P97 luciferase reporter plasmids and eukaryotic expression vectors for E8̂E2C, E2 and E8̂E2C d3-12 or with the parental plasmid pSG5. The average relative luciferase activities were calculated with respect to the activity of each construct in the presence of the parental pSG5 expression vector, which was set to 1. Standard deviations are indicated by the vertical lines above the bars. The structure of the pGL31URR plasmid is shown below the graph. Conserved E2BS1 to -4 and the P97 RNA initiation site are indicated.