Table 1. Target genes, primer sequences, and cycling condition of real-time qPCR.

Target genes	Primers sequences sequence (5′→3′)	Reverse transcription	Primary denaturation	Amplification (40 cycles)			References
				Secondary denaturation	Annealing (optics on)	Extension
B-actin	F: CGTGGGCCGCCCTAGG CACCA	50˚C 30 minutes	94˚C 15 minutes	94˚C 15 seconds	55˚C 30 seconds	72˚C 30 seconds	Fitzpatrick et al. (2002)
	R:GGGGGCCTCGGTCAGC AGCAC
IL-6	F: CCAGCCACAAACACTG ACCT						Wooldridge and Ealy (2019)
	R:TAGCTCTCAGGCTGAA CTGC
INF-γ	F: TGATTCAAATTCCGGTG GATG						Shu et al. (2011)
	R:TTCATTGATGGCTTT GCGC