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. Author manuscript; available in PMC: 2024 Sep 21.
Published in final edited form as: Science. 2024 Aug 2;385(6708):eadk1679. doi: 10.1126/science.adk1679

Fig 2. Distinct gut immune changes after DREADD-mediated neuronal activation.

Fig 2.

(A-F) Quantification of proportions of ileum RORγ+ Tconv (A), ileum neutrophil (B), ileum MHCII+ MNP (C), colon ILC2 (D), colon RORγ+ Treg (E), and cecum RORγ+ Treg (F) with representative flow cytometric plots after chemogenetic activation of different neuronal subsets in distinct ADC mice and controls (CTRL).

(G) Heatmap of average fold changes (relative to CTRL) for significantly changed immune cell populations in the ileum, cecum, and colon from each Cre line compared to control littermates after CNO treatment (P<0.05). White squares are where P>0.05.

Each symbol (A-F) represents an individual mouse; Error bars represent mean and standard deviation. *,P < 0.05; **, P < 0.01 ***; P < 0.001 (unpaired Student’s t test with Holm-Sidak correction for multiple comparison). Data are representative of ≥2 independent experiments. n = 7-12 mice/group.