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Neutralizing antibody specificity generated by C4/89.6-V3 peptide immunization. Serum samples were obtained 2 weeks after final boosting with the C4/89.6-V3 peptide. Each serum sample was incubated for 1 h at 37°C with either no peptide (solid circles), C4/scbl-V3 peptide (open circles), or C4/89.6-V3 peptide (solid inverted triangles) and then assessed for neutralizing activity against SHIV-89.6 in MT-2 cells.
