Figure 6.

A mutagenic pathway linking AVETRTL (WT) and variants WVATRIW and WVVTRAF.A, a mutagenic pathway for a WVVTRAF variant. The amino acid residues for the WT and the WVATRIW variant are indicated in the top right-hand corner. Residues that were mutated in the WT to reach the sequence of this variant are underlined. Each variant is presented as a node and the respective residues that differ in WT and a particular variant are indicated. Nodes are shaded according to their relative hemolytic activity. The possible mutagenic pathways are indicated by red lines. B, relative hemolytic activity of WVATRIW variants with additional mutations at conserved positions V403 and T460. C, a mutagenic pathway for a WVVTRAF variant. All details are as in (A).