FIG. 6.

Evaluation of the efficiency and receptor specificity of Ad5LucFF/6H-mediated gene transfer. (A) Specificity of Ad5LucFF/6H binding to the AR. 293/6H cells grown in monolayer culture were preincubated with various concentrations of either the truncated form of fibritin or fibritin carrying a carboxy-terminal six-His tag, fibritin-6H, prior to infection with Ad5LucFF/6H. Luciferase activities detected in the lysates of infected cels 20 h postinfection are given as percentages of the activity in the absence of blocking protein. (B) Gene transfer to 293 and 293/6H cells. Cells seeded in 24-well plates were infected with various doses of Ad5Luc1 or Ad5LucFF/6H. The MOI was equal to 40, 400, or 4,000 virus particles per cell. Twenty hours postinfection, the cells were collected and lysed, and the luciferase activities of the lysates were measured in relative light units (rlu). On both graphs, each data point was set in triplicate and calculated as the mean of three determinations. The error bars show standard deviations.