Fig. 2.

Pretreatment with AMOLE prevented H₂O₂-induced toxicity in HepG2 cells through suppression of oxidative stress. HepG2 cells were pretreated with AMOLE (125–500 μg mL⁻¹; 24 h), then exposed to H₂O₂ (500 μM; 1 h). (A) The cell viability following treatment was observed with MTT assay (B) The intracellular oxidative status was evaluated by DCFH-DA assay. The cell viability and oxidative status following treatment were calculated relative to the untreated control (n = 3; mean ± SEM; *, p < 0.05; ****, p < 0.0001 v.s. untreated control; ^††, p < 0.01; ^†††, p < 0.001; ^††††, p < 0.0001 v.s. H₂O₂ group).