REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD45 (clone H130, purified)	Biolegend	Cat#304002
Anti-CD31 (clone WM59, purified)	Biolegend	Cat#303102
Anti-CD57 (clone HCD57, conjugated to 142Nd)	Standard BioTools	Cat#3142007B
Anti-CD38 (clone HIT2, purified)	Biolegend	Cat#303502
Anti-CD8 (clone SK1, purified)	Biolegend	Cat#344727
Anti-CD4 (clone RPA-T4, conjugated to 145Nd)	Standard BioTools	Cat#3145001B
Anti-IgD (clone IA6-2, conjugated to 146Nd)	Standard BioTools	Cat#3146005B
Anti-CXCR3 (clone G025H7, purified)	Biolegend	Cat#353702
Anti-CD16 (clone 3G8, conjugated to 148Nd)	Standard BioTools	Cat#3148004B
Anti CD127 (clone A019D5, conjugated to 149Sm)	Standard BioTools	Cat#3149011B
Anti-OX40 (clone ACT35, conjugated to 150Nd)	Standard BioTools	Cat#3150023B
Anti-CCR6 (clone G034E3, purified)	Biolegend	Cat#353402
Anti-TCR-γδ (clone 11F2, conjugated to 152Sm)	Standard BioTools	Cat#3152008B
Anti-CCR4 (clone L291H4, purified)	Biolegend	Cat# 359402
Anti-CD73 (clone AD2, purified)	Biolegend	Cat# 344002
Anti-PD1 (clone EH12.2H7, conjugated to 155Gd)	Standard BioTools	Cat#3155009B
Anti-CD45RA (clone HI100, purified)	Biolegend	Cat#304102
Anti-CD33 (clone WM53, conjugated to 158Gd)	Standard BioTools	Cat#3158001B
Anti-CD161 (clone HP-3G10, conjugated to 159Tb)	Standard BioTools	Cat#3159004B
Anti-CD39 (clone A1, conjugated to 160Gd)	Standard BioTools	Cat#3160004B
Anti-ICOS (clone C398.4A, purified)	Biolegend	Cat# 313502
Anti-CD27 (clone L128 conjugated to 167Er)	Standard BioTools	Cat#3167006B
Anti-CD56 (clone NCAM16.2, conjugated to 163Dy)	Standard BioTools	Cat#3163007B
Anti-CD95 (clone DX2, conjugated to 164Dy)	Standard BioTools	Cat#3164008B
Anti-CD19 (clone HIB19, conjugated to 165Ho)	Standard BioTools	Cat#3165025B
Anti-CD24 (clone ML5, conjugated to 166Er)	Standard BioTools	Cat#3166007B
Anti-CCR7 (clone G043H7, purified)	Biolegend	Cat#353202
Anti-CXCR5 (clone J252D4, purified)	Biolegend	Cat#356902
Anti-CD25 (clone 2A3 conjugated to 169Tm)	Standard BioTools	Cat#3169003B
Anti-CD123 (clone 6H6, purified)	Biolegend	Cat# 306002
Anti-CD5 (clone UCHT2, purified)	Biolegend	Cat# 300602
Anti-CD11c (clone 3.9, purified)	Biolegend	Cat#301602
Anti-CD3 (clone UCHT1, purified)	Biolegend	Cat#300402
Anti-HLA-DR (clone L243, conjugated to 174Yb)	Standard BioTools	Cat# 3174001B
Anti-CD14 (clone M5E2, conjugated to 175Lu)	Standard BioTools	Cat# 3175015B
Anti-TCRva7.2 (clone 3C10, purified)	Biolegend	Cat#351702
Anti-CD11b (clone ICRF44, conjugated to 209Bi)	Standard BioTools	Cat#3209003B
Anti-TCR gamma-delta 2 (clone 123R3, purified)	Miltenyi	Custom request
Anti-CX3CR1 (clone 2A91, purified)	Biolegend	Cat#341602
Anti-TIGIT (clone A15153G, purified)	Biolegend	Cat#372702
Anti-CD45RA (clone HI100, purified)	Biolegend	Cat#304102
Anti-NKp30 (clone Z25, conjugated to 159Tb)	Standard BioTools	Cat#3159017B
Anti-TCR-delta 1 (clone REA173, purified)	Miltenyi	Cat#130-122-285
Anti-NKp46 (clone BAB281, conjugated to 162Dy)	Standard BioTools	Cat#3162021B
Anti-NKG2C-PE (clone 134591)	R&D Systems	Cat#FAB138P
Anti-PE (clone PE001, conjugated to 165Ho)	Standard BioTools	Cat#3165015B
Anti-NKG2D (clone ON72, conjugated to 166Er)	Standard BioTools	Cat#3166016B
Anti-NKG2A (clone Z199, conjugated to 169Tm)	Standard BioTools	Cat#3169013B
Anti-CD161 (clone HP-3G10, purified)	Biolegend	Cat#339902
Anti-DNAM (clone DX11, conjugated to 171Yb)	Standard BioTools	Cat#3171013B
Anti-CD3-e450 (clone SK7)	Thermo Fisher	Cat#48-0036-42
Anti-CD56-APC (clone CMSSB)	Thermo Fisher	Cat# 17-0567-42
Anti-CD16-PE-Cy7 (clone eBioCB16)	Thermo Fisher	Cat# 25-0168-42
Anti-CD57-PerCP-Cy5.5 (clone QA17A04)	Biolegend	Cat#393312
Anti-CD107a-FITC (clone H4A3)	Biolegend	Cat#328606
Anti-TNFa-AF700 (clone MAB11)	Biolegend	Cat# 502928
Anti-IFNg-PE (clone 4S.B3)	Biolegend	Cat#502510
Anti-CD19-APC-Cy7 (clone HIB19)	Biolegend	Cat#302218
Anti-CD14-APC-Cy7 (clone 63D3)	Biolegend	Cat#367108
Anti-Perforin-PE-Cy7 (clone dG9)	Thermo Fisher	Cat#25-994-42
Anti-GranzymeB-PE-Texas-Red (clone GB11)	Invitrogen	Cat#10558363
Anti-CCR2 (clone K036C2, purified)	Biolegend	Cat#357202
Anti-CD57 (clone HNK-1, purified)	Biolegend	Cat# 359602
Anti-CD36 (clone 5-271, purified)	Biolegend	Cat# 336202
Anti-CD69 (clone FN50, conjugated to 144Nd)	Standard BioTools	Cat#3144018B
Anti-KIR2DL1/S1/S3/S5 (clone HP-MA4, purified)	Biolegend	Cat#339502
Anti-CXCR1 (clone 42705, purified)	R&D Biosystems	Cat#MAB330
Anti-CXCR2 (clone 5E8/CXCR2, conjugated to 147Sm)	Standard BioTools	Cat#3147010B
Anti-CD14 (Clone RMO52, conjugated to 148Nd)	Standard BioTools	Cat#3148010B
Anti-CD25 (clone 2A3, conjugated to 149Sm)	Standard BioTools	Cat#3149010B
Anti-CD27 (clone LG.3A10, conjugated to 150Nd)	Standard BioTools	Cat#3150017B
Anti-KIR2DL1/S5 (clone 143211, purified)	R&D Biosystems	Cat# MAB1844
Anti-ILT1 (clone 24, purified)	Biolegend	Cat#337902
Anti-CXCR5 (clone RF8B2, conjugated to 153Eu)	Standard BioTools	Cat#3153020B
Anti-TIGIT (clone MBSA43, conjugated to 154Sm)	Standard BioTools	Cat#3154016B
Anti-TIM3 (clone F38-2E2, conjugated to 154Sm)	Standard BioTools	Cat#3154010B
Anti-CD56 (clone B159, conjugated to 155Gd)	Standard BioTools	Cat#3155008B
Anti-ILT2 (clone GHI/75, conjugated to 156Gd)	Standard BioTools	Cat#3156020B
Anti-ILT2/LIR6 (clone 586326, purified)	R&D Biosystems	Cat#MAB30851
Anti-NKp80 (clone 239127, purified)	R&D Biosystems	Cat#MAB1900
Anti-CXCR6 (clone K041E5, conjugated to 160Gd)	Standard BioTools	Cat#3160016B
Anti-ILT3 (clone ZM4.1, purified)	Biolegend	Cat#333002
Anti-ILT5 (clone 222821, purified)	R&D Biosystems	Cat#MAB1806
Anti-ILT4 (clone 42D1, conjugated to 161Dy)	Standard BioTools	Cat#3161019B
Anti-NKp44 (clone P44-8, purified)	Biolegend	Cat#325102
Anti-CXCR4 (clone 12G5, purified)	Biolegend	Cat#306502
Anti-CXCR3 (clone G025H7, conjugated to 163Dy)	Standard BioTools	Cat#3163004B
Anti-CD161 (clone HP3G10, conjugated to 164Dy)	Standard BioTools	Cat#3164009B
Anti-KIR2DS4 (clone 179315, purified)	R&D Biosystems	Cat#MAB1847
Anti-NKG2D (clone ON72, conjugated to 166Er)	Standard BioTools	Cat#3166016B
Anti-KIR3DL1 (clone DX9, conjugated to 167Er)	Standard BioTools	Cat#3167013B
Anti-KIR3DL2 (clone 539304, purified)	R&D Biosystems	Cat# MAB2878
Anti-CD127 (clone A019D5, conjugated to 168Er)	Standard BioTools	Cat#3168017B
Anti-NKG2A (clone Z199, conjugated to 169Tm)	Standard BioTools	Cat#3169013B
Anti-CD122 (clone Tu27, conjugated to 170Er)	Standard BioTools	Cat#3170004B
Anti-CD226 (clone DX11, conjugated to 171Yb)	Standard BioTools	Cat#3171013B
Anti-CX3CR1 (clone 2A9-1, purified)	Biolegend	Cat#341602
Anti-KIR2DL2/L3 (clone DX27, conjugated to 173Yb)	Standard BioTools	Cat#3173010B
Anti-KIR2DL5 (clone UP-R1, purified)	Miltenyi	Cat#130-096-199
Anti-CD94 (clone HP-3D9, conjugated to 174Yb)	Standard BioTools	Cat#3174015B
Anti-PD1 (clone EH12.2H7, conjugated to 175Lu)	Standard BioTools	Cat#3175008B
Anti-KIR2DL4 (clone 181703, purified)	R&D Biosystems	Cat#MAB2238
Anti-KIR2DL2/L3/S2/S4 (clone 180704, purified)	R&D Biosystems	Cat#MAB1848
Anti-CD16 (clone 3G8, conjugated to 209Bi)	Standard BioTools	Cat#3209002B
Biological samples
PBMC, serum and plasma from healthy children.	N/A	N/A
Chemicals, peptides, and recombinant proteins
Maxpar X8 Antibody Labelling kits (various isotopes)	Fluidigm	various
Maxpar MCP9 Antibody Labelling kits (various isotopes)	Fluidigm	various
EQ four element calibration beads	Fluidigm	Cat#201078
Trustain Fc receptor blocking solution	Biolegend	Cat#422302
Cell-ID iridium intercalator	Fluidigm	Cat#201192B
Cell Staining Media (CSM, phosphate buffered saline + 0.5% foetal bovine serum + 0.02% sodium azide)	Prepared in house	N/A
Deposited data
Mass cytometry datafiles (antibody panels 1 and 2).	Mendeley Data	https://doi.org/10.17632/dhfp3wzc77.1
Software and algorithms
Prism	Graphpad
Cytobank Cytometry Analysis Software	Cytobank
R (version 3.5.3)	www.R-project.org
Other
Helios Mass Cytometer	Fluidigm	N/A
LSRFortessa X-20 Cytometer	Beckton Dickinson	N/A