TABLE 1.
Oligodeoxynucleotide primers
| Oligodeoxynucleotide | Sequence | NADL nucleotide positions |
|---|---|---|
| 183 | (+)-TTTTCTAGATAATACGACTCACTATAGTATACGAGAATTAGAAAAGGCACTCGa | 1–27 |
| 344 | (−)-GTCTTTTGGAGGTGAACGACTGCCCC | 5810–5835 |
| 378 | (+)-CATGGCACATGGGGCCCGCCGTGTGTAAGAAGb | 378–388, 5423–5443 |
| 379 | (−)-CACGGCGGGCCCCATGTGCCATGTACAGCAGAGb | 368–388, 5423–5434 |
| 886 | (+)-CCAGAAGGTACCCCATTGTATc | 517–537 |
| 887 | (−)-ACGGCGGGCCCCATATTATCATCGTGTcd | 386–399, 643–655 |
| 888 | (+)-TCTCTGCTGTACATGGCACATGGGACCTGCTGTTTGTAAAAAAATCACAGe | 367–416 |
| 889 | (−)-TCTGCTGTACATGGCACATGGGGCCTGCCGTGTGTAAGe | 369–406 |
a
Oligodeoxynucleotide primer 183 has been described previously (19).
b
The ApaI site and silent nucleotide substitution (T394C) are indicated by underlining and bold lettering, respectively.
c
The numbers for nt 517 to 537 for primer 886 and nt 643 to 655 of primer 887 refer to 5′ BVDV plus EMCVdelB3ABC (9).
d
The numbers for nt 386 to 399 for primer 887 refer to MetNS3, with 386 being the A of the AUG start codon.
e
The nucleotide numbers for mutant primers 888 and 889 refer to the MetNS3 sequence, with 386 being the A of the AUG start codon.