FIG. 1.
Features of the JS7 cell line. (A) Confluent monolayer showing the polygonal, epithelial appearance of JS7 cells and presence of perinuclear granules (arrows) (bar = 20 μm). (B) Transmission electron micrograph showing ultrastructural features of JS7 cells including surface microvilli (mv), desmosomes (arrows), and intracytoplasmic lamellar body-like structures (arrowheads) (bar = 10 μm). (C) Protein immunoblot analysis using goat antiserum to Mason-Pfizer monkey virus p27 and iodinated rabbit anti-sheep F(ab)2 to detect the 26-kDa JSRV CA (arrowhead) in lung fluid from a natural case of OPC (lane a) and in supernatants from a JS7 cell culture, passage 8 (lane b). Immunoglobulin light chain (faint fuzzy band above the JSRV CA band in lung fluid [lane a]) is absent in preparations from the JS7 culture supernatant.