FIG. 3.

CMV-based JSRV_JS7 plasmid constructs. Schematic representation of the full-length JSRV_JS7 provirus clone in which the 5′ U3 region was replaced by the CMV promoter inserted 5′ to the JSRV_JS7 RNA start site. The insert shows the context of the region joining the CMV promoter with the 5′ R region of JSRV_JS7. Also shown are pCMV-J:gag-pol and pCMV-J:env. Both of these constructs were designed to overexpress viral proteins by using the CMV promoter to drive transcription. Standard retrovirus notation is used. These constructs were transfected into 293T cells for production of JSRV virions.