Table 3.

Box–Behnken design matrix for the three independent variables and their corresponding experimental observed responses.

Run	A	B	C	Biomass (1010CFU/ml)
1	10	25	15	1.67
2	10	30	10	1.55
3	15	20	15	1.65
4	10	25	15	1.67
5	10	25	15	1.70
6	10	30	20	1.49
7	5	25	20	1.80
8	5	20	15	1.87
9	10	20	20	1.65
10	5	30	15	1.72
11	10	25	15	1.72
12	15	25	10	1.75
13	5	25	10	1.79
14	10	25	15	1.68
15	10	20	10	1.66
16	15	30	15	1.67
17	15	25	20	1.70

A: glucose; B: yeast extract; C: MgSO4.