Figure 5.

Effects of BFstatin on the gene expression of V. vulnificus. (A) The V. vulnificus JN111 genes differentially expressed by BFstatin are visualized in a volcano plot. The gray dashed lines represent cutoffs for differential expression of |fold change| >2 and adjusted p-value <0.05. The blue and red dots indicate the genes differentially down-regulated and up-regulated by the addition of 20  μM BFstatin, respectively. (B) Expression fold changes of the regulatory and structural genes of the BrpR regulon in V. vulnificus JN111 treated with BFstatin, ΔbrpR treated with DMSO, and ΔbrpR treated with BFstatin relative to those in JN111 treated with DMSO are displayed as a heatmap. The comparative analyses of the gene expression were performed with biological triplicates.