FIG. 3.

(A) Expression of CAR in various tissues of adult (5-month-old) transgenic mice. Samples (10 μg of protein each) of tissue dissolved in Laemmli sample buffer were loaded in each lane of an SDS–10% PAGE gel. Proteins in the gel were transferred to nitrocellulose and incubated with antiserum to the extracellular domain of mouse CAR. From left to right, the samples were from tibialis anterior (TA), quadriceps (Q), soleus (S), diaphragm (D), heart (H), lung (Lg), liver (L), and spleen (Sp) tissue. Numbers on the left indicate the position of molecular weight markers included in a flanking lane. (B) Comparison of the level of expression of CAR in tibialis anterior muscle from a 5-month-old transgenic mouse (lane TA) with expression in brain of a 10-day-old control, nontransgenic littermate (lane B). Samples (5 μg of protein each) of tissue dissolved in Laemmli sample buffer were loaded in each lane of an SDS–10% PAGE gel. Proteins in the gel were transferred to nitrocellulose and incubated with antiserum to the extracellular domain of mouse CAR. Numbers on the right of the panel indicate the position of molecular weight markers included in a flanking lane.