FIG. 5.

AdV transducibility of CAR transgenics by AdV expressing lacZ under the control of the CMV enhancer–β-actin promoter. Quantitation of the number of β-galactosidase-positive fibers following a single 25-μl injection of either 1 × 10¹² or 2 × 10¹¹ particles/ml into the tibialis anterior muscle of 5- to 6-month-old hemizygous CAR transgenic mice compared to control, nontransgenic littermates. At the higher dose, differences between the control group and the CAR transgenics were significant (P < 0.001; ANOVA). Note that transducibility obtained with the CAR transgenics injected with the lower dose is similar to that with controls receiving the higher dose of AdV (differences between these two groups are not significant by ANOVA, followed by the Bonferroni posttest). Results are means ± standard error.