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Reduction in MIA PaCa-2 cell-derived spheroids. (A) Spheroids after treatment with 0 or 10 mM sodium citrate in medium with the indicated glucose concentrations for 72 h, analyzed using an inverted microscope. Scale bar: 100 μm (B) The relative change in spheroid size (n = 3 in each of the three independent experiments) was estimated using Olympus CellSens software. The data are expressed as the mean ± SD. **p < 0.01. MIA PaCa-2, a pancreatic cancer cell line; SD, standard deviation.
