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. 2024 Sep 11;10(18):e37226. doi: 10.1016/j.heliyon.2024.e37226

Fig. 2.

Fig. 2

Polyphyllin I induced apoptosis of HCT8 cells. A. HCT8 cells treated with 5 μM PPI for 24 h were analyzed by flow cytometry. B. The percentage of apoptotic HCT8 cells. C. HCT8 cells were treated with 5 μM PPI for 24 h, and the protein levels of Bax and Bcl-2 were detected by Western blot analysis. D. Quantification of Bax and Bcl-2 expression levels in HCT8 cells using Image-Pro Plus 6.0 software. Data were presented as the means ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs Control.