Skip to main content
. 2024 Sep 11;10(18):e37226. doi: 10.1016/j.heliyon.2024.e37226

Fig. 3.

Fig. 3

Polyphyllin I induced cell cycle arrest in HCT8 cells. A. Under 5 μM PPI treatment for 24 h, flow cytometry was used to analyze the distribution percentages of different phases of the HCT8 cell cycle. B. The percentage of cells at different phases of the cycle was displayed as a bar graph. C. Under 5 μM PPI treatment for 24 h, the expressions of cell cycle-regulated proteins were measured by Western blot. D. Quantification of P53 and CDK2 expression levels in HCT8 cells with Image-Pro Plus 6.0 software. Data were presented as the means ± SD, n = 3, ***P < 0.001 vs Control.