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Polyphyllin I downregulated the expressions of Notch signaling pathway-related proteins in HCT-8 cells. A. After treating HCT8 cells with 5 μM PPI for 24 h, the expression levels of Notch1, Jagged1, and Hes1 in cells were detected via Western blot. β-Actin was employed as an internal control. B-D. Protein expressions were evaluated via histogram analyses. Data were presented as the means ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs Control.
